Tween 20 CAS 9005-64-5 Polysorbate 20

Factory Supply Tween 20 CAS 9005-64-5 Polysorbate 20 with Best Price

  • Appearance: Yellow liquid
  • Purity: 99. 0%min
  • Stock: In stock
  • Sample: Available
  • Zhishang Chemical: Tween 20 Supplier & Manufacturer

Basic Info

What is Tween 20?

Tween 20, whose usual trade names include Alkest TW 20 as well as polysorbate 20, is a light non-ionic surfactant developed by ethoxylation of dehydrated sorbitol before adding lauric acid, which is a medium chain fatty acid primarily existing in coconut oil. Due to its security and fairly non-toxic residential or commercial properties, it is allowed to be made use of as an emulsifier, cleaning agent, dispersant, solvent, and stabilizer in various fields such as medication, chemical industry, food, and also fabrics.

Tween 20 is widely made use of in the fields of biotechnology as well as scientific research, and can be added to immunohistochemistry barriers and also reagents, such as Western blots and ELISAs, to assist avoid non-specific antibody binding, minimize history discoloration, and improve reagent diffusion. Additionally, Tween 20, as a common food quality additive, has likewise been applied in food production, as well as its visibility is currently present in numerous consumables on the market. It can likewise be made use of as a flavoring mouthdrop (such as Ice Drops), oral or non stomach suspension, and as a wetting representative in rubber product packaging machines in the elastomer sector.

Tween 20 Uses

  1. Tween 20 is a non-ionic surfactant. It is used as an oil-in-water emulsifier, and can be used as a solvent, diffusing agent, stabilizer, lubricant and antistatic agent. Gas chromatography stationary liquid (maximum use temperature 120°C) for separation and analysis of volatile oils, fatty acid esters, alcohols, ketones and halides.
  2. Tween 20 has the function of refolding antigen, which can improve the specific recognition ability. When doing western blot, blocking the unbound sites on the membrane with inert protein or non-ionic detergent can reduce the non-specific binding of antibodies. The blocking agent should block all unbound sites without displacing the target protein on the membrane, not binding to the target protein site, and not cross-reacting with antibodies or detection reagents.
  3. Tween 20 removes detergent, and the concentration should not exceed 0.3% (0.05% works best). If the milk sealing effect is not good, you can increase the Tween concentration.

The Tween in the WB washing solution is the same as BSA, which provides protection to the antigen-antibody protein. At the same time, because it is a surfactant, it can reduce the non-specific effect of the antibody on the antigen, and is used to elute unbound antibodies and reduce non-specificity. combined. Tween 20 is when we use TBST, the dosage of Tween 20 is 0.5ml/L, without adding SDS.

Tween 20 can be prepared by condensing sorbitol and its mono-anhydrous, double-anhydrous compounds and lauric acid esters with about 20 moles of ethylene oxide per mole of sorbitol and its dehydrated compounds under alkaline conditions. Its solubilization and diffusion are mainly used in the synthesis.

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This is William, CEO of Zhishang Chemical Co., Ltd.

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Application of Tween 20

Difference between Tween 80 and Tween 20

There are three differences between Tween 80( also called polysorbate 80) and Tween 20


  1. Tween 20 – WikiPedia
  2. Study on the Recovery of Protein During the Extraction of Peanut Oil by Tween-Assisted Aqueous Method

Tween 20 Supplier and Manufacturer

As a tween 20 supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best tween 20 raw materials to customers all over the world.

Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.

If you have a demand for tween 20 and related products, please contact our service staff Zhishang Chemical – White directly, and we will provide you with high-quality products at the best price.

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