
Tween 20 CAS 9005-64-5 Polysorbate 20



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- Appearance: Yellow liquid
- Purity: 99. 0%min
- Stock: In stock
- Sample: Available
- Zhishang Chemical: Tween 20 Supplier & Manufacturer
Tween 20: The Complete Guide
Tween 20 for Sale
Basic Info
Chemical Name: | Tween 20 |
Other Name: | Polysorbate 20; Tween surfactant; Polyethylene glycol sorbitan monolaurate |
CAS: | 9005-64-5 |
EINECS: | 500-018-3 |
Type: | Food additives; Pharmaceutical raw materials; Organic raw materials; Cosmetic raw materials; Surfactants |
Molecular Formula: | C26H50O10 |
Molecular Weight: | 522.6692 |
Melting point | 98.9 °C (decomp) |
Boiling point | 100 °C |
density | 1.11 g/mL at 20 °C |
vapor pressure | <1.4 hPa (20 °C) |
refractive index | n20/D 1.468(lit.) |
Fp | >230 °F |
storage temp. | Store below +30°C. |
solubility | 100 g/L |
form | viscous liquid |
Specific Gravity | 1.090~1.130 (20/20℃) |
color | Amber |
Brand Name: | Zhishang Chemical |
Provide: | Tween 20 MSDS; Tween 20 COA |
What is Tween 20?
Tween 20, whose usual trade names include Alkest TW 20 as well as polysorbate 20, is a light non-ionic surfactant developed by ethoxylation of dehydrated sorbitol before adding lauric acid, which is a medium chain fatty acid primarily existing in coconut oil. Due to its security and fairly non-toxic residential or commercial properties, it is allowed to be made use of as an emulsifier, cleaning agent, dispersant, solvent, and stabilizer in various fields such as medication, chemical industry, food, and also fabrics.
Tween 20 is widely made use of in the fields of biotechnology as well as scientific research, and can be added to immunohistochemistry barriers and also reagents, such as Western blots and ELISAs, to assist avoid non-specific antibody binding, minimize history discoloration, and improve reagent diffusion. Additionally, Tween 20, as a common food quality additive, has likewise been applied in food production, as well as its visibility is currently present in numerous consumables on the market. It can likewise be made use of as a flavoring mouthdrop (such as Ice Drops), oral or non stomach suspension, and as a wetting representative in rubber product packaging machines in the elastomer sector.
Tween 20 Uses
- Tween 20 is a non-ionic surfactant. It is used as an oil-in-water emulsifier, and can be used as a solvent, diffusing agent, stabilizer, lubricant and antistatic agent. Gas chromatography stationary liquid (maximum use temperature 120°C) for separation and analysis of volatile oils, fatty acid esters, alcohols, ketones and halides.
- Tween 20 has the function of refolding antigen, which can improve the specific recognition ability. When doing western blot, blocking the unbound sites on the membrane with inert protein or non-ionic detergent can reduce the non-specific binding of antibodies. The blocking agent should block all unbound sites without displacing the target protein on the membrane, not binding to the target protein site, and not cross-reacting with antibodies or detection reagents.
- Tween 20 removes detergent, and the concentration should not exceed 0.3% (0.05% works best). If the milk sealing effect is not good, you can increase the Tween concentration.
The Tween in the WB washing solution is the same as BSA, which provides protection to the antigen-antibody protein. At the same time, because it is a surfactant, it can reduce the non-specific effect of the antibody on the antigen, and is used to elute unbound antibodies and reduce non-specificity. combined. Tween 20 is when we use TBST, the dosage of Tween 20 is 0.5ml/L, without adding SDS.
Tween 20 can be prepared by condensing sorbitol and its mono-anhydrous, double-anhydrous compounds and lauric acid esters with about 20 moles of ethylene oxide per mole of sorbitol and its dehydrated compounds under alkaline conditions. Its solubilization and diffusion are mainly used in the synthesis.
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Application of Tween 20
Polysorbate 20 is used as a wetting down agent in spicing mouthdrops similar as Ice Drops, helping to give a sense of prolixity for other factors similar as SD alcohol and mint flavor.
The World Health Organization recommends an respectable diurnal input limit of 0- 25 milligrams of polyoxyethylene dehydrated sorbitol ester per kilogram of body weight.
Polysorbate 20 has a wide range of operations in the fields of biotechnology and wisdom. For illustration, it’s used to:
- As cleansers in immunoassay, similar as Western spots and ELISAs. It helps helpnon-specific antibody list. In this main operation, it’s dissolved in Tris softened saline or phosphate softened saline at a dilution of0.05 to0.5 v/ v. These buffers are used for washing between each vulnerable response to remove footloose vulnerable substances, and eventually for incubating vulnerable reagent( labeled antibody) results to reducenon-specific backgrounds.
- Souse the list spots on the face( i.e., fleece polystyrene microporous plates, generally binding to proteins similar as BSA).
- Stabilize purified protein outgrowth( PPD) result for tuberculosis exposed skin test.
- As a solubilizer for membrane proteins.
- Used to lyse mammalian cells at attention ranging from0.05 to0.5 v/ v, generally used in combination with other cleansers, mariners, and complements.
Polysorbate 20 is used as an excipient in medicinal operations to stabilize embrocation and dormancies.
Numerous brands of baby wipes use polysorbate 20.
Polysorbate 20 is used by stamp collectors to remove prints from envelopes and remove any residue on the prints without damaging the prints themselves.
Polysorbate 20 is also used as a wetting down agent for rubber packaging machines in the elastomer assiduity.
Polysorbate 20 has been used as a shape directing agent to synthesize globular magnetite nano modules.
Difference between Tween 80 and Tween 20
There are three differences between Tween 80( also called polysorbate 80) and Tween 20
Synthetic raw accoutrements of Tween 80 Polymerized from sorbitan monooleate and ethylene oxide.
Synthetic raw material of Tween 20 a admixture of polyoxyethylene sorbitan monolaurate and a part of polyoxyethylene bissorbitan monolaurate.
Use of Tween 80 Tween 80 is a polyol- type nonionic surfactant and is the most generally used one among polysorbates. It has remarkable resistance to electrolytes and strong hydrophilicity. It’s extensively used in liquid,semi-solid and solid medications as O/ W emulsifier, solubilizer, wetting down agent, dispersant and stabilizer.
The use of Tween 20 It’s a condensation product of Span and ethylene oxide. Because there are more hydrophilic groups- polyoxyethylene groups in its patch, it has strong hydrophilicity and is anon-ionic detoxifying agent. Stain agent. It’s frequently used as an oil painting- in- water( O/ W) emulsifier; it can be used in combination with other emulsifiers similar as sodium lauryl sulfate or spans to increase the stability of the conflation. It can also be used as a solubilizer for some medicines.
Parcels of Tween 80 The peroxide value of Tween 80 doesn’t exceed 10, and it’s a light unheroic to orange thick liquid; it has a slight peculiar smell. The taste is slightly bitter and slightly tangy, with a sense of warmth. Soluble in water, ethanol, methanol or ethyl acetate, veritably slightly answerable in mineral oil painting.
Parcels of Tween 20 Tween 20 is unheroic or amber clear unctuous liquid with special odor and faint bitter taste. The relative viscosity is1.01, the boiling point is> 100 °C, the flash point is 321 °C, the refractive indicator is1.472, and the density( 25 °C) is0.25 ~0.40 Pa ·s.
The patch contains further hydrophilic groups, miscible with water, ethanol, methanol and ethyl acetate, undoable in liquid paraffin, fixed oil painting and light petroleum, 1 part of this product can be dissolved in 130 corridor of cottonseed oil painting and 200 corridor corridor of toluene, 5 waterless result pH 5
7; HLB value16.7.
Reference
- Tween 20 – WikiPedia
- Study on the Recovery of Protein During the Extraction of Peanut Oil by Tween-Assisted Aqueous Method
Tween 20 Supplier and Manufacturer
As a tween 20 supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best tween 20 raw materials to customers all over the world.
Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.
If you have a demand for tween 20 and related products, please contact our service staff Zhishang Chemical – White directly, and we will provide you with high-quality products at the best price.
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* Any inquiries will be replied to within 24 hours.
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After-Sales Service
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Do you accept sample order?
We will make samples before mass production, and after the sample is approved, we’ll begin mass production. Doing 100% inspection during production, then doing random inspection before packing.
What’s your MOQ?
Our MOQ is 1kg. But usually we accept less quantities, such as 100g on the condition that the sample charge is 100% paid.
Is there a discount?
The different quantity has different discount.
HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?
You can get free samples for some products, you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests, we will manufacture the products according to your requests.
Do you supply product report?
Yes. We’ll give you product analysis report before shipping.
1. ≤50kg, Express delivery recommended, usually called DDU service;
2. ≤500kg, Air shipping recommended, usually called FOB, CFR, or CIF service;
3. >500kg, sea shipping recommended, usually called FOB, CFR, or CIF service;
4. For high-value products, please select air shipping and express delivery for safe.
Quality Control of Tween 20

