Triton X-100/Triton X-405 CAS 9002-93-1

Cervical liquid-based cytometry technology (TCT) significantly improves the quality of cervical cell preparation, makes the cells evenly distributed on the slide, removes mucus, red blood cells and inflammatory exudates, ensures the integrity of cells, and at the same time. It also preserves the antigenic biological activity of cells, and is often used in immunocytochemical detection. However, in practice, we found that in the process of immunocytochemical smear of TCT, the nuclear and cytoplasmic markers were lower in cell positivity and expression intensity compared with the corresponding tissue slices.

Treating cell sheets with Triton X-100 solved the above problems and obtained satisfactory results. TritonX-100 is a non-ionic surfactant (commonly known as detergent), which can dissolve the cell membrane in immunocytochemistry by dissolving the lipid component on the cell membrane, so that the antibody can enter the cell and combine with the antigen.

After two treatments with Triton X-100, the cell membrane was completely dissolved, and the antibody was fully combined with the corresponding antigen, thereby improving the positive detection rate of cells, and enhancing the blocking effect of hydrogen peroxide and serum, reducing the background. Especially useful in immunocytochemistry. When treating glass slides with TritonX-100, the concentration of 0.3% and the time of 1 min is appropriate for the first time, and the concentration of 0.1% and time of 15 minutes is appropriate for the second time. Too high concentration or too long time will destroy tissue structure, cause background non-specific staining, and also cause delamination.

Heavy oil refers to the crude oil whose viscosity exceeds 100 mPa·s at the formation temperature. Due to its characteristics of high viscosity, high density and poor fluidity, its extraction and transportation are still the key research directions in the future. In addition, my country still focuses on the exploitation of heavy oil at this stage, and its exploitation process is more complicated than conventional crude oil. It is not only required that the viscosity reduction process has high viscosity reduction performance, but also simple operation, environmental protection and high economic value.

At present, there are many viscosity reduction technologies used in China, such as viscosity reduction by mixing thin oil, microbial viscosity reduction, catalytic cracking viscosity reduction, heat treatment viscosity reduction, emulsification viscosity reduction, etc. By comparison, viscosity reduction by mixing thin oil may cause wax deposition and reduce oil quality. , the economy is unreasonable, the development of microbial viscosity reduction is still immature, the catalytic cracking viscosity reduction technology has high requirements, the catalyst products are easy to pollute the environment, the catalytic treatment temperature is high, and the heat treatment viscosity reduction investment and energy consumption are high. It is a viscosity reduction method with better viscosity reduction effect, higher economic value and more environmentally friendly post-processing work in the process.

Triton X-100 (Triton X-100) is a non-ionic surfactant, which does not ionize in aqueous solution and has high stability; Strong dissolving properties. Dodecyldimethylbetaine (BS-12) is a zwitterionic surfactant with high solubility and excellent stability in a wide pH range; in an alkaline aqueous solution environment, BS-12 It will show the characteristics of anionic surfactants, and in the acidic aqueous environment, it will show the characteristics of cationic surfactants.

At the same time, adding a small amount of alkaline reactants to the heavy oil can neutralize the acidic substances in the oil, and react with them to generate natural surfactants, which invert the heavy oil to form an O/W emulsion, thereby reducing the flow of the heavy oil. resistance. In an alkali-free environment, the viscosity of heavy oil decreases gradually with the increase of the mass fraction of Triton X-100.

When the mass fraction of Triton X-100 was 1.6%, the emulsification reduction effect of heavy oil was better, and the viscosity reduction rate was 75.93%. The compounding system of Triton X-100 and BS-12 has a synergistic effect on emulsification and viscosity reduction of heavy oil. The emulsification and viscosity reduction effect of TritonX-100 with a mass fraction of 1.6% is compared with that of BS-12 with a mass fraction of 0.6%. It is better to use Triton X-100 aqueous solution alone, and the viscosity reduction rate is 92.63%. In an alkaline environment, the compound system of Triton X-100 and BS-12 has the best emulsification and viscosity reduction effect on heavy oil, the viscosity reduction rate is 93.14%, and the emulsifier stability is higher.