Triton X-100 CAS 9002-93-1
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- Appearance: Colorless liquid
- Assay: 99. 0%min
- Stock: In stock
- Sample: Available
- Zhishang Chemical: Triton X-100 Suppiler&Manufacturer
Triton X-100: The Complete Guide
Triton X-100 for Sale
Basic Info of Triton X-100
OCTYLPHENYLPOLYETHYLENE GLYCOL; OP-4 Emulsifier; Octylphenol ethoxylate; OPE; Alkylaryl polyether alcohols; Octylphenoxypolyethoxyethanol
Pharmaceutical raw materials; Organic raw materials
What is Triton X-100?
Triton X-100 is a non-ionic surfactant with hydrophilic polyethylene oxide chain (with approximately 9.5 ethylene oxide devices) and aromatic hydrocarbon lipophilic or hydrophobic teams. The hydrocarbon group is 4 – (1,1,3,3-tetramethylbutyl) – phenyl. The Triton X-100 is closely pertaining to IGEPAL CA-630 or the previous Nonidet P-40, and also might be differentiated from it by its somewhat shorter ethylene oxide chain. For That Reason, Triton X-100 is more hydrophilic than Igepal CA-630; For many applications, both cleaning agents may not be thought about functionally compatible.
Triton X-100 was originally a registered hallmark of Rohm and Haas Company, and was consequently obtained by Union Carbide Firm. Dow Chemical Company obtained Union Carbide Company and then acquired it. Not long after (2009 ), Dow likewise obtained Rohm & Haas.
Triton X-100 is a typical non-ionic surfactant as well as emulsifier, generally made use of in biochemical applications to dissolve proteins. It is considered to be a reasonably moderate detergent, non denaturing, as well as has been reported in lots of referrals as a standard enhancement reagent. It is utilized to split cells to essence proteins and also organelles. It can also penetrate the living cell membrane layer for makeover.
Triton X-100 Uses
Triton-X 100 is made use of as a nonionic cleaning agent, emulsifier as well as dispersant.
Triton X-100 is a non-ionic surfactant that does not dissociate in water, has high stability in solution, is not easily affected by strong electrolyte inorganic salts, and can combine with lipids such as phospholipids in biological membranes to form Soluble complex; the hydrophobic end can also bind to the hydrophobic region of the membrane protein to form a complex that dissolves in solution.
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Application of Triton X-100
Cervical liquid-based cytometry technology (TCT) significantly improves the quality of cervical cell preparation, makes the cells evenly distributed on the slide, removes mucus, red blood cells and inflammatory exudates, ensures the integrity of cells, and at the same time. It also preserves the antigenic biological activity of cells, and is often used in immunocytochemical detection. However, in practice, we found that in the process of immunocytochemical smear of TCT, the nuclear and cytoplasmic markers were lower in cell positivity and expression intensity compared with the corresponding tissue slices.
Treating cell sheets with Triton X-100 solved the above problems and obtained satisfactory results. TritonX-100 is a non-ionic surfactant (commonly known as detergent), which can dissolve the cell membrane in immunocytochemistry by dissolving the lipid component on the cell membrane, so that the antibody can enter the cell and combine with the antigen.
After two treatments with Triton X-100, the cell membrane was completely dissolved, and the antibody was fully combined with the corresponding antigen, thereby improving the positive detection rate of cells, and enhancing the blocking effect of hydrogen peroxide and serum, reducing the background. Especially useful in immunocytochemistry. When treating glass slides with TritonX-100, the concentration of 0.3% and the time of 1 min is appropriate for the first time, and the concentration of 0.1% and time of 15 minutes is appropriate for the second time. Too high concentration or too long time will destroy tissue structure, cause background non-specific staining, and also cause delamination.
Triton X-100 is a frequently utilized cleaner busy. Triton X-100 is widely made use of to split cells to extract healthy proteins or organelles, or to pass through the membrane layers of living cells.Some applications consist of:
- Inactivation of lipid layered infections (such as HIV, HBV, HCV) in biopharmaceutical production
- Industrial use (steel electroplating)
- Components in flu injections, consisting of Fluarix, Flublok and Fluzone
- Penetrate unfixed (or a little dealt with) eukaryotic cell membrane
- Liquify natural membrane healthy protein along with zwitterionic detergent such as CHAPS
- Partial lysis barrier in DNA extraction (generally in 5% solution in alkaline lysis barrier)
- Decrease the surface area tension of liquid remedy throughout immunostaining (normally 0.1-0.5% in TBS or PBS barrier)
- Dispersion of Carbon Products for Soft Composites
- Restricting colony growth of Aspergillus nidulans in microbiology
- Acellular cells of pet beginning
- Remove SDS from SDS-PAGE gel before renaturation of healthy proteins in gel
- Damage cell monolayer as favorable control for TEER measurement.
- Minimize surface stress in etching, such as damaging fine functions (micron dimension openings) in MEMS gadget processing
- It is a component of Image Flo, which is a service made use of for photographic handling to prevent moisture from transferring on the movie after drying out
In addition to research laboratory usage, the Triton X-100 can be used in a selection of cleaning representatives, from heavy commercial products to moderate cleaners. It, together with pure water and also isopropanol, is also a typical active ingredient in homemade vinyl document cleaning option.
Triton X-100 Suppliers and Manufacturers
As a triton x-100 supplier and manufacturer with strict standard product quality system certificate, Zhishang Chemical has long been providing the best triton x-100 raw materials to customers all over the world.
Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.
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