Sodium L-ascorbyl-2-phosphate CAS 66170-10-3

L-ascorbic acid-2-phosphate trisodium salt can be used as alkaline phosphatase catalytic substrate. Alkaline phosphatase (ALP) is a vital biomarker whose unusual expression is related to numerous illness such as bust cancer, prostate cancer cells, bone disease, diabetic issues, as well as liver dysfunction. Measurable detection of ALP in product can aid in the diagnosis of related diseases. Spectroscopic evaluation has actually been extensively utilized in ALP discovery because of its simplicity as well as quick response. However, conventional spectroscopic methods based on a single material and with a single colorimetric or fluorescent output signal have poor anti-interference ability and low accuracy, and are difficult to detect targets in complex samples. With the advancement of nanotechnology, a variety of ALP colorimetric sensing methods based on nano-gold and nano-silver have been developed, but the sensitivity of such methods needs to be improved; the weak shell changes of core-shell nanoparticles can cause significant Spectral and color variations, which appear to offer the possibility to further increase the sensitivity of colorimetric detection. Some studies have provided a dual-channel detection method for alkaline phosphatase activity. The principle is that alkaline phosphatase catalyzes the hydrolysis of its substrate L-ascorbic acid-2-phosphate trisodium salt (AAP) to generate reducing ascorbic acid, which reduces ascorbic acid. Tollen’s reagent generates silver element and deposits on the surface of gold nanoparticles to form Au@AgNPs, which leads to the change of solution color and absorption spectrum; in addition, the generated Au@AgNPs can effectively quench the fluorescence of graphene quantum dots (GQDs), resulting in the change of solution color and absorption spectrum. Changes in fluorescence intensity. Thus, colorimetric and fluorescence dual-channel determination of alkaline phosphatase activity can be achieved through simultaneous changes in solution color, absorbance, and fluorescence intensity.

L-ascorbic acid-2-phosphate trisodium salt can also be used for the preparation of a photoelectrochemical parathion sensor. It belongs to the technical field of novel nanometer functional materials and biosensors. First, a new two-dimensional nanophotosensitive material Mn-MoO3/TiO2@g-C3N4 was prepared. Taking advantage of the good biocompatibility and large specific surface area of the material, parathion antibody was loaded, and then the glutaraldehyde Alkaline phosphatase is immobilized by cross-linking. During detection, since alkaline phosphatase can catalyze L-ascorbic acid-2-phosphate trisodium salt AAP to generate L-ascorbic acid AA in situ, and then provide electron donors for photoelectric detection, The effect of the specific quantitative binding of antibody and antigen on the electron transport ability is used to reduce the photocurrent intensity accordingly. Finally, a label-free detection of parathion with low cost, high sensitivity, good specificity, rapid detection and simple preparation is obtained. photoelectrochemical biosensors.