Is R-Phycoerythrin suitable for multicolor flow cytometry?

Yes. R-PE is a core standard reagent for multicolor flow cytometry panels. Its 565 nm absorption peak matches perfectly with common 488 nm and 561 nm instrument lasers. It can be formulated into tandem conjugates including PE-Cy5 and PE-Cy7 to extend the emission spectrum using only one excitation laser. Ensure your flow cytometer optical filters align with the corresponding emission peaks: 575 nm for native PE, 670 nm and above for tandem fluorophores.

R-PHYCOERYTHRIN CAS 11016-17-4 Supplier

Q: What is the distinction between R-PE and B-PE?

R-PE is extracted from red algae and has a distinct absorption spectrum (peaks at 496, 545, and 565 nm) and molecular structure. B-PE is derived from different algal sources with slightly different spectral properties. R-PE is the most widely used variant for flow cytometry applications.

Q: How should R-Phycoerythrin be stored?

Store at 2–8 °C inside a sealed, light-shielded container. Do not freeze the product, as freezing will cause irreversible aggregation and permanent loss of fluorescence intensity. When preserved as a 60% ammonium sulfate suspension, it remains stable for multiple months under proper storage conditions. Any diluted or desalted solutions should be used promptly after preparation.

Q: How do I conjugate R-PE to antibodies?

R-PE requires chemical activation via crosslinkers such as SMCC or NHS ester prior to antibody conjugation. Activated R-PE is incubated with target antibody (typically at a 1:1 molar ratio) at pH 7.2–7.4 for 1–2 hours at room temperature. Always follow a validated labeling protocol to retain maximum brightness of the final conjugate. Commercial ready-to-use conjugation kits are also available for streamlined workflows.

R-Phycoerythrin red fluorescent protein CAS 11016-17-4 zhishangchemical

Surfactant Appearance: POWDER
Proportion: Minimum 99.0%
Inventory: In stock
Sample: In stock
Zhishang Chemical: Supplier and manufacturer of R-PHYCOERYTHRIN CAS 11016-17-4

Basic Info of R-PHYCOERYTHRIN CAS 11016-17-4

R-PHYCOERYTHRIN Chemical Properties

storage temp.	2-8°C
form	ammonium sulfate suspension
color	pink to red or purple
Cosmetics Ingredients Functions	SKIN PROTECTING

What is R-PHYCOERYTHRIN ?

R-Phycoerythrin (R-PE, CAS 11016-17-4) is a bright red, water-soluble fluorescent protein from the phycobiliprotein family. It is extracted from red algae, and its framework contains alpha (20,000 Da), beta (20,000 Da), and gamma (30,000 Da) subunits with covalently attached bilin chromophores. The healthy protein has a molecular weight of around 240 kDa

Key Applications

Flow Cytometry:

R-PHYCOERYTHRIN is one of the most extensively utilized fluorescent dye in flow cytometry. It is delighted at 488 nm and 561 nm, offering a lot stronger fluorescence than FITC– frequently 5 to 10 times brighter on a per-molecule basis. It is particularly useful for spotting low-abundance antigens (e.g., CD25, CD127). In multicolor panels, R-PHYCOERYTHRIN is often coupled with FITC and APC as distinct networks.

Antibody Labeling:

As a protein,R-PHYCOERYTHRIN needs to be chemically triggered to develop responsive teams (like NHS esters or maleimide) before conjugation to antibodies. Commercial sets are offered for straight labeling. The resulting antibody-PE conjugates are utilized in immunofluorescence, immunohistochemistry, and ELISA applications.

Tandem Dye Applications:

R-PHYCOERYTHRIN features as a power benefactor in tandem dye conjugates (e.g., PE-Cy5, PE-Cy7). In these conjugates, the R-PHYCOERYTHRIN moves its energy to an additional fluorophore, enabling signal discovery at longer wavelengths using a single excitation line. This increases the variety of observable criteria in multicolor analysis.

Product Packaging

Why Select zhishangbio as Your R-Phycoerythrin Distributor?

Quality and Supply Chain

We provide R-Phycoerythrin with ≥ 95% pureness. Each batch consists of a complete Certification of Analysis covering focus (≥ 10 mg/mL), purity (A550/A280 ratio commonly ≥ 5.0), and spectral properties. The product is provided in a 60% ammonium sulfate suspension with 100 mM sodium phosphate, 100 mM NaCl, and 2 mM salt azide, pH 7.0. This formulation keeps stability during transport and storage space. The product is shipped at ambient temperature and ought to be refrigerated upon invoice. It has to be shielded from light in any way times.

Stock and Technical Support

We maintain supply at our central stockroom. Basic pack dimensions: 1mg, 5mg, and 25mg [v] Smaller sized amounts ship within 1-2 company days. Bulk orders ship within 5-7 company days. We likewise provide support for solution guidance– including buffer exchange, desalting, and activation methods for antibody conjugation. For mass or custom-made orders, call our sales group for quantity discounts and long-lasting supply agreements.

FAQ

What is the distinction between R-PE and B-PE?

R-PHYCOERYTHRIN is removed from red algae and has a distinct absorption spectrum (peaks at 496, 545, and 565 nm) and molecular structure. B-PE is from various algal resources with a little various spooky residential or commercial properties. R-PE is the most generally previously owned form in circulation cytometry.

Exactly how should R-Phycoerythrin be stored?

Store at 2-8 ° C in a secured, light-protected container. Do not ice up, as this can create irreparable gathering and loss of fluorescence. As a 60% ammonium sulfate suspension, it is secure for numerous months under proper conditions. Diluted or desalted options must be made use of quickly.

How do I conjugate R-PE to antibodies?

R-PHYCOERYTHRIN calls for chemical activation (with SMCC or NHS ester) before conjugation. The active R-PE is then nurtured with antibody (commonly 1:1 molar proportion) at pH 7.2-7.4, 1-2 hours at room temperature. Comply with a confirmed labeling protocol to keep the illumination of the conjugate. Business conjugation packages are also available.

Is R-Phycoerythrin suitable for multicolor circulation cytometry?

Yes. R-PHYCOERYTHRIN is a basic reagent in multicolor panels. Its 565 nm excitation height sets well with 488 nm and 561 nm lasers. It can be utilized in tandem conjugates (PE-Cy5, PE-Cy7) to prolong the discharge array while making use of a solitary excitation source. Make sure instrument filters match the discharge tops (575 nm for PE, 670 nm+ for tandem dyes).

References

R-PHYCOERYTHRIN CAS 11016-17-4 – PubChem

The Manager has Something to Say

If you want to buy R-PHYCOERYTHRIN, pls contact us to get a free quote.

This is William, CEO of Zhishang Chemical Co., Ltd.

Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

R-PHYCOERYTHRIN Supplier and Manufacturer

As a R-PHYCOERYTHRIN supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best R-PHYCOERYTHRIN raw materials to customers all over the world.

Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.

If you have a demand for R-PHYCOERYTHRIN and related products, please contact our service staff Zhishang Chemical – White directly, and we will provide you with high-quality products at the best price.