Propidium Iodide CAS 25535-16-4 | DNA Staining Reagent Supplier
98% / 99% High Purity Propidium Iodide CAS 25535-16-4
- Appearance:powder
- Proportion: Minimum 99.0%
- Inventory: In stock
- Sample: Free sample
- Zhishang Chemical: Supplier and manufacturer of Propidium Iodide CAS 25535-16-4
Description of Propidium Iodide
Propidium Iodide Properties and Applications
Propidium iodide (PI), CAS 25535-16-4, is a fluorescent nucleic acid dye widely used in life science research, cell biology, flow cytometry, fluorescence microscopy and laboratory analysis. It is an organic iodide salt with the molecular formula C27H34I2N4 and a molecular weight of approximately 668.4 g/mol. Public chemical references describe Propidium iodide as a fluorescent dye that binds to nucleic acids, with an absorbance maximum around 535 nm and an emission maximum around 617 nm.
One of the most useful features of Propidium Iodide CAS 25535-16-4 is its relationship with cell membrane integrity. Because PI generally does not cross intact plasma membranes efficiently, it can enter cells with compromised membranes and bind to nucleic acids, producing a measurable fluorescent signal. This property makes Propidium iodide staining widely useful for cell viability analysis and live/dead cell discrimination. Reviews of flow cytometry methods describe PI and related DNA-binding dyes as established tools for identifying cells with compromised membranes.
PI is also commonly used in cell cycle analysis. When cells are appropriately prepared and stained, the amount of PI-associated fluorescence can be used to estimate cellular DNA content by flow cytometry. Published laboratory protocols use PI staining together with RNase treatment to analyze DNA-content distributions corresponding to the G0/G1, S and G2/M phases of the cell cycle.
Main Applications of Propidium Iodide
1. Cell Viability Analysis
Propidium iodide for cell viability is one of the most established research applications. PI can enter cells with compromised membranes and bind nucleic acids, allowing researchers to identify a population with reduced membrane integrity using fluorescence-based analysis.
2. Flow Cytometry
Propidium iodide for flow cytometry is widely used in laboratory protocols for viability assessment and DNA-content analysis. Its fluorescence can be detected using suitable flow-cytometry equipment, making PI a practical reagent for cell population analysis.
3. Cell Cycle Analysis
PI is frequently used for cell cycle analysis by flow cytometry. With appropriate sample preparation and RNase treatment, PI fluorescence can be used to estimate cellular DNA content and distinguish populations associated with G0/G1, S and G2/M phases.
4. DNA and Nuclear Staining
Propidium iodide DNA staining is another established laboratory application. PI binds nucleic acids and can be used in fluorescence-based research involving DNA content and nuclear staining. Sigma-Aldrich documents applications including DNA staining, cell and nucleus staining, and flow cytometry.
5. Fluorescence Microscopy
Propidium iodide can also be used with suitable fluorescence microscopy and confocal microscopy workflows. Commercial PI solutions are documented for fluorescence microscopy, confocal microscopy, flow cytometry and fluorometry applications.
| Property | Information |
|---|---|
| Product Name | Propidium Iodide |
| Abbreviation | PI |
| CAS Number | 25535-16-4 |
| Molecular Formula | C27H34I2N4 |
| Molecular Weight | 668.4 g/mol |
| Chemical Type | Organic Iodide Salt |
| Appearance | Powder |
| Main Function | Fluorescent Nucleic Acid Dye |
| DNA Binding | Yes |
| Main Research Areas | Cell Biology, Flow Cytometry, Molecular Biology |
| Main Applications | Cell Viability, DNA Staining, Cell Cycle Analysis |
| Absorbance Maximum | Approximately 535 nm |
| Emission Maximum | Approximately 617 nm |
| Typical Storage | Grade/form dependent; follow product specification |
| Documentation | COA / SDS / Product Specification |
Propidium Iodide 's supply advantage and supply capability
For buyers sourcing Propidium Iodide 25535-16-4, Zhishang Chemical focuses on the practical requirements that matter in laboratory purchasing: consistent specifications, batch control, documentation, packaging and international supply.
Our laboratory and quality-control system supports chemical raw-material testing and product verification. Zhishang Chemical states that its laboratory covers more than 1,500 square metres and includes analytical equipment such as HPLC, GC, FTIR, NMR and AAS. The company also reports a technical team including PhDs and master’s-level specialists.
For Propidium iodide powder, quality control is especially important because research applications can be sensitive to product purity and fluorescence performance. Before shipment, the applicable product specification and batch information can be checked, while documents such as COA and SDS/MSDS can be provided according to the order requirements.
The packaging of Propidium Iodide
We will use different standard packaging according to the product form.
● Liquid product: packaged in blue plastic drums, with a standard specification of 200 kg/drum; 220kg/barrel/250kg/barrel, tightly sealed barrel mouth, leak proof, resistant to bumps, suitable for long-distance transportation.
● Powdered products: packaged in thick cardboard drums, with a standard net weight of 25 kilograms per drum; 200 kg/barrel; 220kg/barrel/250kg/barrel, the barrel body is sturdy and pressure resistant, while also having good moisture resistance.
● Sample: sealed uniformly in aluminum foil bags with strong sealing abilities, which can prevent moisture and air from entering the bag and cause it to deteriorate.
Packaging can be tailored to meet customer needs, with standard packaging processes that ensure the quality of goods is maintained during storage and transport.
Propidium Iodide Supplier and Manufacturer
As a Propidium Iodide supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best Propidium Iodide raw materials to customers all over the world.
Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.
If you have a demand for Propidium Iodide and related products, please contact our service staff Zhishang Chemical – White directly, and we will provide you with high-quality products at the best price.
Supply Plan and Delivery Schedule for Propidium Iodide
| Scope | Standard Order | Bulk Order | Urgent Order |
|---|---|---|---|
| MOQ | 1kg | Negotiable per product | 1 kg minimum |
| Supply Capacity | Regular & stable supply | Arranged per production schedule | Priority stock allocation |
| Lead‑time for Stock Preparation | 1‑3 working days | 3‑10 working days | Response within 24 hours |
| Production Cycle | 3‑7 working days | 7‑20 working days | Subject to stock availability |
| Shipping Lead‑time | 3‑7 working days | Per order schedule | Within 24‑48 hours |
| Shipping Method | Air / Sea / Express | Air / Sea | Air / Express |
| Packaging | Standard packaging | Customizable | Standard stock packaging |
| Target Customers | Regular procurement | Long‑term / large‑volume buyers | Customers for urgent restocking |
Delivery commitment:
We will arrange production, quality inspection, packaging, and logistics in a timely manner after order confirmation. The specific delivery date is subject to order confirmation, production progress, and actual shipping schedule.
✏️For regular products, we will prioritize inventory and production planning;
✏️For customized products, a special supply plan will be developed based on technical requirements and production cycles.
References
Our laboratory&certificate patents
Zhishang Chemical has its own R&D lab, with professional teams and advanced equipment. We also have rigorous processes and procedures to ensure the quality of our products.
▶ The laboratory is over 1500 square metres in size, and has been divided into functional zones to accommodate testing requirements.
▶ The core R&D Team consists of three PhDs and seven or more masters with rich technical expertise.
▶ Equipment with international advanced analytical and testing equipment, such as HPLC GC, FTIR NMR AAS etc. to achieve precise control during the entire process.
▶ We can develop customized products and will optimize formulas and processes according to customer needs.
▶ Set strict standards for product quality inspection and make sure that each batch is subjected to multiple inspection stages in order to ensure stable and reliable performance.
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FAQ:
Propidium iodide (PI) is a fluorescent nucleic acid dye mainly used in cell viability analysis, flow cytometry, DNA staining and cell cycle analysis. Because PI is generally excluded by intact cell membranes and can enter membrane-compromised cells, it is commonly used for live/dead cell discrimination.
In suitable flow-cytometry assays, Propidium iodide enters cells with compromised plasma membranes and binds nucleic acids, producing a fluorescent signal that can be detected by the instrument. When used for DNA-content analysis, PI fluorescence can provide information about the relative DNA content of individual cells.
Yes. Propidium iodide cell cycle analysis is an established flow-cytometry application. After appropriate fixation and sample preparation, PI staining can be combined with RNase treatment to measure cellular DNA content and analyze G0/G1, S and G2/M populations.
Before purchasing Propidium Iodide 25535-16-4, buyers should confirm the required assay, physical form, fluorescence specifications, packaging, storage conditions, COA, SDS/MSDS and intended application. If the material is being used for flow cytometry or another sensitive research method, it is useful to confirm that the supplier’s specification matches the laboratory’s established protocol.
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