Phenyl Isothiocyanate CAS 103-72-0
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- Appearance: Colorless to yellow liquid
- Purity: 99. 0%min
- Stock: In stock
- Sample: Available
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Phenyl Isothiocyanate: The Complete Guide
Phenyl Isothiocyanate for Sale
|Chemical Name:||Phenyl Isothiocyanate|
|Other Name:||Benzene-1-isothiocyanate; Fenylisothiokyanat; PITC|
|Type:||Organic intermediate; Organic raw materials|
What is Phenyl Isothiocyanate?
Phenyl isothiocyanate (PITC) is a reagent utilized for reverse stage HPLC. PITC is not as delicate as o-phthalaldehyde (OPA) as well as can not be fully automated. Unlike OPA, PITC can be used to analyze secondary amines. Additionally referred to As Edman reagent, it is made use of for Edman degradation.
Phenyl isothiocyanate is the favored reagent for automatic Edman destruction system. Nonetheless, this reagent is very poisonous. For hands-on modification, various other reagents are chosen, such as dimethylaminoazobenzene isothiocyanate (Chang, 1983; Wang et al. isothiocyanate (Bartlet-Jones et al., 1994). The last reagent has the advantage of being volatile, so it can be conveniently removed by vacuum prior to MS evaluation (Spengler, 1997). The compound was successfully utilized in 7 successful hand- operated pyrolysis cycles and also MS analysis. Nevertheless, the high sensitivity of these substances seems to show synthetic adjustment, such as the “acetylation” of the hydroxyl teams of Ser and also Thr. Allyl isothiocyanate showed much better selectivity.
It is acquired by response of diphenylthiourea as well as hydrochloric acid. Diphenylthiourea is contributed to hydrochloric acid to heat and dissolve, as well as the created phenyl isothiocyanate is distilled. In the distillate oashil, equivalent volume of water to Chemical, contributed to and the water layer is divided, and then cleaned with weakened alkali solution. After a big quantity of water washing, dry with anhydrous calcium chloride as well as collect at 120-121 ℃ (4.66 kPa), which is the ended up item.
Phenyl Isothiocyanate Uses
- Amino acid derivative reagent, ultraviolet detection. Derivation is completed in 5-10 minutes, and excess reagent is removed by distillation.
- Phenyl isothiocyanate is a type of isothiocyanate ester with phenyl linked to nitrogen; Utilized for amino acid sequencing in Edman destruction. It has the function of irritant as well as reagent.
- Phenyl isothiocyanate is a fragrant isothiocyanate. It joins the dehydration reaction of alcohol. It is commonly made use of to manufacture various heterocyclic substances with crucial organic value.
- Phenyl isothiocyanate is made use of as a derivative reagent of key as well as additional amines. It is used for peptide sequencing by Edman degradation and amino acid evaluation by HPLC. It is made use of to derivate N-terminal amino acids of healthy proteins for automated sequence evaluation. It is a synthon of dithiodiazazafulvalene.
- Phenyl isothiocyanate – WikiPedia
- F. B. Dains, R. Q. Brewster, and C. P. Olander. “Phenyl isothiocyanate”.
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