o-Phthalaldehyde CAS 643-79-8

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  • Appearance: Yellow powder
  • Purity: 99. 0%min
  • Stock: In stock
  • Sample: Available
  • Zhishang Chemical: o-Phthalaldehyde Supplier & Manufacturer

Basic Info

What is o-Phthalaldehyde?

O-phthalaldehyde (ortho-phthaldialdehyde) is a pharmaceutical intermediate, which has actually been commonly used in amine alkaloids, fluorometer histamine resolution as well as pharmaceutical inspection. The finished item is light yellow needle-like crystal, sol uble in water, alcohol, ether and other natural solvents, has no poor smell, and has steady chemical residential properties. It was first made use of for endoscope sanitation in 1994 as well as was located to have a broad-spectrum as well as high-efficiency antiseptic result. Succeeding researches have shown that o-phthalaldehyde has actually numerous advantages compared to glutaraldehyde. As a new sort of high-efficiency chemical disinfectant for outside usage, o-phthalaldehyde has passed the US FDA qualification in 1999.

At present, the vast majority of medical instruments (endoscopes, surgical instruments, dental instruments, etc.) at home and abroad are sterilized by glutaraldehyde solutions, including enhanced glutaraldehyde solutions, compound glutaraldehyde solutions, and the like. However, the solution is inconvenient to use, requires activation before use, strong irritation, strong protective ventilation, long inactivation time for bacteria and viruses, and strong corrosion to sterilized metal instruments. In view of these circumstances, people have been looking for a substitute that excels in all aspects.

Phthalaldehyde solution is a high-level disinfectant for reprocessing heat-sensitive medical equipment. In nearly 30 years, o-phthalaldehyde solution was the first new high-level disinfectant with remarkable material compatibility compared to glutaraldehyde. The o-phthalaldehyde solution can exert rapid and high-level disinfection within 12 minutes at room temperature (20°C), and is especially effective against mycobacteria (including M.chelona, which is resistant to glutaraldehyde). Phthalaldehyde solutions are “ready to use”, non-activating, virtually odorless products.

o-Phthalaldehyde Uses

Yellow needle-like crystal, primarily used in medication, dyes, and so on.Amino acid derivatization reagent, fluorescence detection, the reaction can be finished in one min, but the product is unsteady as well as requires to be detected immediately.

o-Phthalaldehyde is a pharmaceutical intermediate, the latest high-efficiency as well as safe antibacterial disinfectant for outside use, utilized for sanitation as well as sterilization of endoscopic medical tools in medical facilities, and can be used to synthesize new anti-platelet aggregation medication indopofen, as well as is additionally an analytical reagent in the chemical field.

Pre-column derivatization reagent for primary amines and amino acids. Fluorescent derivatives can be detected by reverse phase HPLC. This reaction requires OPA, primary amines and thiols. Amines can be quantified in the presence of excess sulfhydryl groups. In the presence of excess amine, sulfhydryl groups can be quantified.

Phthalaldehyde has been used in:

  • Preparation of o-phthalaldehyde reagent for analysis of gentamicin content.
  • Preparation of reagents for the determination of the degree of hydrolysis of milk proteins.
  • Free amino acids in milk samples were measured by the o-phthalaldehyde/N-acetyl-L-cysteine (OPA/NAC) assay.
  • In the derivatization of putrescine samples.

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Application of o-Phthalaldehyde

Phthalaldehyde has a strong killing effect on bacterial propagules, fungi, mycobacteria, viruses, and spores. The effect of o-phthalaldehyde on killing Bacillus subtilis black variety (C9372) spores, Candida albicans (AT0C10231), Escherichia coli (8099), Staphylococcus aureus (ATCC6538) and Pseudomonas aeruginosa PA01 was tested. In the carrier bactericidal test, 5000mg/L o-phthalaldehyde acts on Escherichia coli and Staphylococcus aureus for 3 minutes, and acts on Candida albicans for 5 minutes, so that the amount of the three bacteria decreases by more than 6log.

In the suspension test, the 18rng/L o-phthalaldehyde solution can kill more than 5log of Pseudomonas aeruginosa after 20 minutes of action. When the concentration of o-phthalaldehyde is 180mg/L for 5 minutes, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa can all be reduced by more than 5log, while glutaraldehyde needs 450mg/L to achieve the same killing effect. However, o-phthalaldehyde has relatively weak spore-killing ability under neutral conditions. In the spore killing test of 5000mg/L bacillus spores, the logarithm of killing is only 0.09; but when the pH is adjusted to 8, 4959mg/L of phthalaldehyde is used for 240min, and 5969mg/L of phthalaldehyde is used for 120min , the logarithms of killing Bacillus subtilis spores on the stainless steel carrier reached 3.69 and 5.0 respectively, indicating that the killing effect of o-phthalaldehyde on tooth cells was significantly improved under alkaline conditions.

It has also been found that o-phthalaldehyde has a faster killing effect on mycobacteria, especially on glutaraldehyde-resistant Mycobacterium chelonae. O-phthalaldehyde with a concentration of 5000mg/L acted on them for 10min and 20min, respectively, to kill the two glutaraldehyde-resistant strains of Mycobacterium chelonae Epping and Mycobacterium Hardidd with a killing effect of more than 6log. The effect of formaldehyde on Mycobacterium tuberculosis can reach more than 5log after 5 minutes of action, while 15000mg/L of glutaraldehyde needs to act on these Mycobacterium chelonis for 32 minutes to achieve the same killing effect.

The U.S. Environmental Protection Agency has identified duck HBV as an indicator virus strain for human HBV inactivation. The duck HBV inactivation carrier experiment was carried out with o-phthalaldehyde, and the result was that 3000 mg/L o-phthalaldehyde could effectively kill duck hepatitis B virus at 20°C for 5 minutes.

Bactericidal Mechanism

Different from the bactericidal mechanism of general disinfectants, the bactericidal activity of o-phthalaldehyde is based on its cross-linking with amino acids and proteins. Studies have shown that o-phthalaldehyde forms a strong cross-linked bond with the bacterial cell wall, forming a barrier that prevents these substances from destroying the structure of the cell wall/membrane. However, the sealing and anti-seepage effect of o-phthalaldehyde also causes the dysfunction of material exchange inside and outside the bacteria, so that the normal physiological functions of bacteria cannot be carried out, thereby promoting cell death.

On the other hand, the form of phthalaldehyde in water is different from that of glutaraldehyde. Phthalaldehyde exists in the solution as a single molecule, so the free aldehyde group can fully play the role of disinfection, while dialdehyde molecules are polymerized by aldehyde group Form existence, the acidity and alkalinity of the solution and other factors affect its degree of polymerization. The degree of polymerization is different, the number of exposed free aldehyde groups is different, and the number of free aldehyde groups determines its disinfection effect. Therefore, the bactericidal effect of o-phthalaldehyde is better than that of glutaraldehyde. Since o-phthalaldehyde is an aromatic aldehyde, it has good fat solubility and is more likely to penetrate the cell membrane of Mycobacterium tuberculosis and Gram-negative bacteria with more lipids, thereby acting on the target site inside the bacteria to cause rapid cell death, so o-phthalaldehyde Phthalaldehyde is stronger than glutaraldehyde in killing Mycobacterium tuberculosis and Gram-negative bacteria.

The spores are more resistant to the outside world than the bacterial propagules. This is because an outer layer structure containing a large amount of dipicolinic acid is formed in the later stage of spore maturation. It is found that o-phthalaldehyde mainly interacts with the amino acids on the spore shell. This cross-linking forms a lethal hard coat that prevents the spores from germinating. Therefore, o-phthalaldehyde kills Bacillus subtilis spores not by destroying DNA, but by inactivating spore germination. effect, and the degree of destruction of phthalaldehyde on the spore germination process is irreversible.


  1. Phthalaldehyde – WikiPedia
  2. Research progress of phthalaldehyde disinfectant

o-Phthalaldehyde Supplier and Manufacturer

As a o-phthalaldehyde supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best o-phthalaldehyde raw materials to customers all over the world.

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If you have a demand for o-phthalaldehyde and related products, please contact our service staff Zhishang Chemical – White directly, and we will provide you with high-quality products at the best price.

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