Guanidine Thiocyanate CAS 593-84-0 GITC
Factory Supply Guanidine Thiocyanate CAS 593-84-0 GITC with Best Price
- Appearance: White powder
- Purity: 99. 0%min
- Stock: In stock
- Sample: Available
- Zhishang Chemical: Guanidine Thiocyanate Supplier & Manufacturer
Guanidine Thiocyanate: The Complete Guide
Guanidine Thiocyanate for Sale
GITC; Guanidinium thiocyanate; Guanidine isothiocyanate; Thiocyanate de guanidinium
Pharmaceutical raw materials; Inorganic salt
What is Guanidine Thiocyanate?
Guanidine thiocyanate is also called Guanidinethiocyanatesalt, chemical name: imidothiocyanate, and its molecular formula is: (NH2)2C: NH·HSCN (118.2).
Guanidine isothiocyanate is in the form of white crystals, the melting point is 116～121℃, the pH value of 6mol/L in water is 4.5～6 at 20℃, and the solubility is completely dissolved. It is a kind of uncoupler and a kind of strong protein denaturant, which can dissolve protein, make protein secondary structure disappear, cell structure degrade, and nucleoprotein is rapidly separated from nucleic acid. For denaturing and lysing cells; extract RNA and DNA, without RNAse and DNAse activity.
Guanidine thiocyanate can be used as a pharmaceutical intermediate. It is used for degreasing in oil industry, as strong lipid degrading agent in daily chemical industry, and cell membrane rupture agent in biopharmaceutical.
Guanidine Thiocyanate Uses
- Guanidine thiocyanate mostly utilized for biomedicine, chemical reagents, etc.
- Chaotropic agent and solid denaturant for denaturing and lysing cells; extracting RNA and DNA.
- Guanidine thiocyanate is a denaturant typically utilized in RNA seclusion. It is utilized as a storage barrier for whole blood samples.Guanidine thiocyanate inactivates nucleases as well as is ideal for storing as well as cold stool examples for DNA research study. Used in mix with phenol-chloroform in RNA removal.
- Guanidine thiocyanate is used for degreasing in oil sector, as solid lipid degrading agent in daily chemical industry, and cell membrane layer rupture agent in biopharmaceutical.
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Application of Guanidine Thiocyanate
Hou Xiaofeng from the Department of Biochemistry, PLA General Hospital conducted the methodological evaluation and performance verification of the guanidine thiocyanate inhibition method for the detection of lactate dehydrogenase isoenzyme 1 (LDH1). Methods: The imprecision, linearity analysis, recovery experiment and normal reference range of guanidine thiocyanate inhibition assay for LDH1 were evaluated.
Results: The intra-assay imprecisions of high, medium and low value samples were 4.25%, 1.57% and 0.97%, respectively, and the inter-assay imprecisions were 4.57%, 2.55% and 1.20%, respectively; the dilution robustness of the linear analysis was good, R2 =0.998; the average recovery rate was 101.56%; there was no significant difference in LDH1 levels between genders; the normal reference range was 33.11±5.76U/L.
Conclusion: The guanidine thiocyanate inhibition method for the detection of LDH1 is reliable and has good performance, which meets the laboratory requirements and can be widely used in clinical practice.
The Preparation Method of Guanidine Thiocyanate
A kind of preparation method of guanidine thiocyanate, has following technological process:
- Put guanidine carbonate and ammonium thiocyanate in a weight ratio of 2:1 into a reaction kettle, and react at 50-90 DEG C for 2 hours to obtain a crude guanidine thiocyanate product.
- Dissolve the crude guanidine thiocyanate in water at a ratio of 1:1 at room temperature.
- Filtration to remove impurities such as raw materials and reaction by-products by filtration.
- Dehydration, the filtered mother liquor is dehydrated at high temperature.
- Crystallization, cooling the supersaturated solution, and concentrating the crystallization to obtain high-purity guanidine thiocyanate.
- Guanidine Thiocyanate – PubChem
- CN201110146840.5 Total RNA preparation method without genomic DNA
- Hou Xiaofeng, Li Juan, Guo Guanghong.Methodological evaluation of the determination of lactate dehydrogenase isoenzyme 1 by guanidine thiocyanate inhibition method[J].Label Immunoassay and Clinical,2015,22(08):798-799+810.
Guanidine Thiocyanate Supplier and Manufacturer
As a guanidine thiocyanate supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best guanidine thiocyanate raw materials to customers all over the world.
Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.
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