Guanidine Thiocyanate CAS 593-84-0 GITC
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Factory Supply Guanidine Thiocyanate CAS 593-84-0 GITC with Best Price
- Appearance: White powder
- Purity: 99. 0%min
- Stock: In stock
- Sample: Available
- Zhishang Chemical: Guanidine Thiocyanate Supplier & Manufacturer
Guanidine Thiocyanate: The Complete Guide
- Item 1: Basic Info
- Item 2: What is Guanidine Thiocyanate?
- Item 3: Guanidine Thiocyanate Uses
- Item 4: The Manager has Something to Say
- Item 5: Application of Guanidine Thiocyanate
- Item 6: The Preparation Method of Guanidine Thiocyanate
- Item 7: Quality Control of Guanidine Thiocyanate
- Item 8: Hot Sale Products
- Item 9: Get Guanidine Thiocyanate Quotation
Guanidine Thiocyanate for Sale
Basic Info
Basic Info
Chemical Properties
Other Info
Basic Info
Chemical Name: | Guanidine Thiocyanate |
Other Name: | GITC; Guanidinium thiocyanate; Guanidine isothiocyanate; Thiocyanate de guanidinium |
CAS: | 593-84-0 |
EINECS: | 209-812-1 |
Type: | Pharmaceutical raw materials; Inorganic salt |
Molecular Formula: | CH5N3.CHNS |
Molecular Weight: | 118.16 |
Chemical Properties
Melting point | 120-122 °C(lit.) |
density | 1.103 g/mL at 20 °C |
refractive index | n20/D 1.482 |
storage temp. | 20-25°C |
solubility | H2O: 6 M at 20 °C, clear, colorless |
form | Crystals or Crystalline Powder |
Specific Gravity | 1.152 |
color | White or colorless |
Odor | Odorless |
PH | 4.8-6.0 (1420g/l, H2O, 20℃)(saturated solution) |
PH Range | 4.8 – 6.0 |
Water Solubility | Soluble in water and ethanol |
Sensitive | Light Sensitive |
Stability: | Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents. |
Other Info
Brand Name: | Zhishang Chemical |
Provide: | Guanidine Thiocyanate MSDS; Guanidine Thiocyanate COA |
What is Guanidine Thiocyanate?
Guanidine thiocyanate is also called Guanidinethiocyanatesalt, chemical name: imidothiocyanate, and its molecular formula is: (NH2)2C: NH·HSCN (118.2).
Guanidine isothiocyanate is in the form of white crystals, the melting point is 116～121℃, the pH value of 6mol/L in water is 4.5～6 at 20℃, and the solubility is completely dissolved. It is a kind of uncoupler and a kind of strong protein denaturant, which can dissolve protein, make protein secondary structure disappear, cell structure degrade, and nucleoprotein is rapidly separated from nucleic acid. For denaturing and lysing cells; extract RNA and DNA, without RNAse and DNAse activity.
Guanidine thiocyanate can be used as a pharmaceutical intermediate. It is used for degreasing in oil industry, as strong lipid degrading agent in daily chemical industry, and cell membrane rupture agent in biopharmaceutical.
Guanidine Thiocyanate Uses
- Guanidine thiocyanate mostly utilized for biomedicine, chemical reagents, etc.
- Chaotropic agent and solid denaturant for denaturing and lysing cells; extracting RNA and DNA.
- Guanidine thiocyanate is a denaturant typically utilized in RNA seclusion. It is utilized as a storage barrier for whole blood samples.Guanidine thiocyanate inactivates nucleases as well as is ideal for storing as well as cold stool examples for DNA research study. Used in mix with phenol-chloroform in RNA removal.
- Guanidine thiocyanate is used for degreasing in oil sector, as solid lipid degrading agent in daily chemical industry, and cell membrane layer rupture agent in biopharmaceutical.
The Manager has Something to Say
This is William, CEO of Zhishang Chemical Co., Ltd.
Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.
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If you want to buy guanidine thiocyanate, pls contact us to get a free quote.
Application of Guanidine Thiocyanate
Application 1
Application 2
Application 1
Hou Xiaofeng from the Department of Biochemistry, PLA General Hospital conducted the methodological evaluation and performance verification of the guanidine thiocyanate inhibition method for the detection of lactate dehydrogenase isoenzyme 1 (LDH1). Methods: The imprecision, linearity analysis, recovery experiment and normal reference range of guanidine thiocyanate inhibition assay for LDH1 were evaluated.
Results: The intra-assay imprecisions of high, medium and low value samples were 4.25%, 1.57% and 0.97%, respectively, and the inter-assay imprecisions were 4.57%, 2.55% and 1.20%, respectively; the dilution robustness of the linear analysis was good, R2 =0.998; the average recovery rate was 101.56%; there was no significant difference in LDH1 levels between genders; the normal reference range was 33.11±5.76U/L.
Conclusion: The guanidine thiocyanate inhibition method for the detection of LDH1 is reliable and has good performance, which meets the laboratory requirements and can be widely used in clinical practice.
Application 2
CN201711222569.2 provides a nucleic acid extraction lysate from exfoliated cells in human feces, comprising: 1mol/L～8mol/L guanidine salt, 1mM～20mM metal ion chelating agent and 100mM-Tris-HCl buffer solution with pH 7.5～8.0, Wherein, the guanidine salt is selected from at least one of guanidine hydrochloride, guanidine isothiocyanate and guanidine thiocyanate, and the metal ion chelating agent is selected from at least one of the water-soluble salts of EDTA and EDTA kind.
Preferably, it also includes 1% by volume to 20% by volume of a surfactant, the surfactant is a non-ionic detergent, and the non-ionic detergent is selected from Tween-20, Tween-80, Brij-35, NP40 and At least one of Triton-100. Related preparation methods and applications are also provided.
The lysing solution of the invention can quickly and efficiently lyse cells, fully release nucleic acid, meet the dosage of subsequent nucleic acid detection, and is suitable for large-scale popularization and application.
The Preparation Method of Guanidine Thiocyanate
Report one
Report two
Report one
A kind of preparation method of guanidine thiocyanate, has following technological process:
- Put guanidine carbonate and ammonium thiocyanate in a weight ratio of 2:1 into a reaction kettle, and react at 50-90 DEG C for 2 hours to obtain a crude guanidine thiocyanate product.
- Dissolve the crude guanidine thiocyanate in water at a ratio of 1:1 at room temperature.
- Filtration to remove impurities such as raw materials and reaction by-products by filtration.
- Dehydration, the filtered mother liquor is dehydrated at high temperature.
- Crystallization, cooling the supersaturated solution, and concentrating the crystallization to obtain high-purity guanidine thiocyanate.
Report two
A preparation method of guanidine thiocyanate is characterized in that having following technological process:
- Put guanidine carbonate and ammonium sulfate in a weight ratio of 2:1 into a reactor, and react at 45-95° C. for 1.5 hours to obtain a crude guanidine thiocyanate product.
- Dissolve the crude guanidine thiocyanate in water at a ratio of 0.5:1 at room temperature.
- Filtration to remove impurities such as raw materials and reaction by-products by filtration.
- Dehydration, the filtered mother liquor is dehydrated at high temperature.
- Crystallize, cool the solution, concentrate and crystallize to obtain high-purity guanidine thiocyanate.
Reference
- Guanidine Thiocyanate – PubChem
- CN201110146840.5 Total RNA preparation method without genomic DNA
- Hou Xiaofeng, Li Juan, Guo Guanghong.Methodological evaluation of the determination of lactate dehydrogenase isoenzyme 1 by guanidine thiocyanate inhibition method[J].Label Immunoassay and Clinical,2015,22(08):798-799+810.
Guanidine Thiocyanate Supplier and Manufacturer
As a guanidine thiocyanate supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best guanidine thiocyanate raw materials to customers all over the world.
Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.
If you have a demand for guanidine thiocyanate and related products, please contact our service staff Zhishang Chemical – White directly, and we will provide you with high-quality products at the best price.
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FAQ
Do you accept sample order?
We will make samples before mass production, and after the sample is approved, we’ll begin mass production. Doing 100% inspection during production, then doing random inspection before packing.
What’s your MOQ?
Our MOQ is 1kg. But usually we accept less quantities, such as 100g on the condition that the sample charge is 100% paid.
Is there a discount?
The different quantity has different discount.
HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?
You can get free samples for some products, you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests, we will manufacture the products according to your requests.
Do you supply product report?
Yes. We’ll give you product analysis report before shipping.
Shipping
1. ≤50kg, Express delivery recommended, usually called DDU service;
2. ≤500kg, Air shipping recommended, usually called FOB, CFR, or CIF service;
3. >500kg, sea shipping recommended, usually called FOB, CFR, or CIF service;
4. For high-value products, please select air shipping and express delivery for safe.
Quality Control of Guanidine Thiocyanate
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