CAS NO 50-01-1 Guanidine hydrochloride

Denaturants (urea and guanidinium hydrochloride) break hydrogen bonds in the protein structure, increasing the solubility of non-polar molecules, including amino acid side chains, reducing hydrophobic interactions; the urea can also penetrate deep into the interior of the protein molecule to affect the protein Close accumulation of molecules. In addition, detergents, organic solvents, heavy metals, heat, mechanical, freezing, ultrasound, high pressure, radiation, etc. can cause protein denaturation. None of these denaturations destroy the covalent bonds of proteins but only involve the destruction of secondary bonds such as hydrogen bonds, salt bonds, hydrophobic interactions and van der Waals interactions. Some denatured proteins automatically return to their natural state when they are removed, a phenomenon known as renaturation of proteins, refolding in protein folding studies.
Inclusion body protein purification
Mostly with strong denaturing agents such as urea (6-8 mol / L) and guanidine hydrochloride (GdnHCl 5-8 mol / L or 6 mol / L) at pH 8, the inclusion bodies are interrupted by ionic interactions In general, guanidine hydrochloride is superior to urea because guanidine hydrochloride is a stronger denaturing agent than urea and dissolves the insoluble inclusion body of urea, and the urea decomposes Isocyanates can result in free carbamoylation of the polypeptide chain, especially when incubated at alkaline pH for a long time. Or with detergents such as SDS, n-hexadecyltrimethylammonium chloride, Sarkosyl, etc., can destroy the hydrophobic bond within the protein, but also can dissolve some of the inclusion body protein. TritonX-100 was used to lyse the Zymononas mobilis levansucrase inclusion body protein. In addition, for cysteine-containing proteins, isolated inclusion bodies typically contain some disulfide bonds formed between the chains and non-reactive disulfide bonds within the chain. There are also organic solvents, alkaline environment (greater than 9) or acid (70% formic acid) can also dissolve.

Product expansion
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