Model No.: CAS NO 50-01-1
Type: Pharmaceutical Intermediates
Appearance: Crystal, Powder
Name:: Guanidine Hydrochloride
MP:: 180-185 °C(lit.)
Density:: 1.18 G/mL At 25 °C(lit.)
Bp:: 132.9ºC At 760 MmHg
Packaging: in 25kg drum
Productivity: 10000kg per month
Place of Origin: Shandong, China (Mainland)
Supply Ability: in stock
Certificate: ISO CE
HS Code: 50-01-1
Denaturants (urea and guanidinium hydrochloride) break hydrogen bonds in the protein structure, increasing the solubility of non-polar molecules, including amino acid side chains, reducing hydrophobic interactions; the urea can also penetrate deep into the interior of the protein molecule to affect the protein Close accumulation of molecules. In addition, detergents, organic solvents, heavy metals, heat, mechanical, freezing, ultrasound, high pressure, radiation, etc. can cause protein denaturation. None of these denaturations destroy the covalent bonds of proteins but only involve the destruction of secondary bonds such as hydrogen bonds, salt bonds, hydrophobic interactions and van der Waals interactions. Some denatured proteins automatically return to their natural state when they are removed, a phenomenon known as renaturation of proteins, refolding in protein folding studies.
Inclusion body protein purification
Mostly with strong denaturing agents such as urea (6-8 mol / L) and guanidine hydrochloride (GdnHCl 5-8 mol / L or 6 mol / L) at pH 8, the inclusion bodies are interrupted by ionic interactions In general, guanidine hydrochloride is superior to urea because guanidine hydrochloride is a stronger denaturing agent than urea and dissolves the insoluble inclusion body of urea, and the urea decomposes Isocyanates can result in free carbamoylation of the polypeptide chain, especially when incubated at alkaline pH for a long time. Or with detergents such as SDS, n-hexadecyltrimethylammonium chloride, Sarkosyl, etc., can destroy the hydrophobic bond within the protein, but also can dissolve some of the inclusion body protein. TritonX-100 was used to lyse the Zymononas mobilis levansucrase inclusion body protein. In addition, for cysteine-containing proteins, isolated inclusion bodies typically contain some disulfide bonds formed between the chains and non-reactive disulfide bonds within the chain. There are also organic solvents, alkaline environment (greater than 9) or acid (70% formic acid) can also dissolve.
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2> Active Pharmaceutical Ingredients(API): API refers to the raw materials in the production of various types of preparations, which is the active ingredient in powder,crystal and paste for medicine use through chemical synthesis,plant extract, and preparations of biotechnology institute.It is one substance or mixture during the medicine making period, it can not be taken directly by patients.
3> Fragrance And Spices: Spice is a substance that can be scented by smelling or scented by taste, and is the raw material for the preparation of the fragrance. Spices are an important part of fine chemicals, it is composed of natural spices, synthetic spices and single fragrance.
4> Organic Raw Material: Intermediates are also known as organic intermediates. The use of coal tar or petroleum products as raw materials to produce dyes, pesticides, pharmaceuticals, resins, additives, and other intermediates.
5> Chemicals Used In Paints: Paint, the traditional Chinese name is paint. So-called coating is coated on the surface of the object that be protected or be adornment, and with the continuous film coating to form strong adhesion, usually in resin, or oil, or emulsion is given priority to, add or not add pigment, filler, add the corresponding additives, organic solvents or water preparation of viscous liquid.