Acetylacetone CAS 123-54-6

Acetylacetone is a commercial bulk chemical with many applications. However, the traditional manufacturing methods have many disadvantages, such as many steps, harsh conditions, low yield, environmental problems and so on, which hinder the further application of petrochemical acetylacetone. Compared with traditional chemical methods, biosynthetic methods have the advantages of environmental friendliness, mild conditions, high selectivity and low potential cost. There is an urgent need to develop a biosynthetic route for acetylacetone to avoid the current problems. Traditionally, acetylacetone is produced by chemical route using acetone and vinyl ketone. Vinyl ketone is produced by pyrolysis of acetone or acetic acid at 700-800 ° C. the by-products are carbon monoxide, methane and hydrogen. Specifically, in the presence of a strong acid catalyst, the esterification reaction of ketene and acetone forms isopropyl acetate (IPA). Then, using metal molybdenum as catalyst, IPA was converted to acetylacetone at 500-600 ° C, with a yield of only about 45%. In a word, this chemical route has many disadvantages, such as many steps, harsh conditions, low yield and environmental problems, which hinder the further application of acetyl acetylacetone. In order to solve this problem, it is necessary to develop new methods for preparing acetylacetone. Compared with traditional chemical methods, biosynthesis is expected to have the advantages of environmental friendliness and mild conditions. IPA is converted to acetylacetone with metal molybdenum as catalyst at 500-600 ° C, and the yield is only about 45%. In a word, this chemical route has many disadvantages, such as many steps, harsh conditions, low yield and environmental problems, which hinder the further application of acetyl acetylacetone. In order to solve this problem, it is necessary to develop new methods for preparing acetylacetone. Compared with traditional chemical methods, biosynthesis is expected to have the advantages of environmental friendliness and mild conditions. IPA is converted to acetylacetone with metal molybdenum as catalyst at 500-600 ° C, and the yield is only about 45%. In a word, this chemical route has many disadvantages, such as many steps, harsh conditions, low yield and environmental problems, which hinder the further application of acetyl acetylacetone. In order to solve this problem, it is necessary to develop new methods for preparing acetylacetone. Compared with traditional chemical methods, biosynthesis is expected to have the advantages of environmental friendliness and mild conditions. In order to solve this problem, it is necessary to develop new methods for preparing acetylacetone. Compared with traditional chemical methods, biosynthesis is expected to have the advantages of environmental friendliness and mild conditions. In order to solve this problem, it is necessary to develop new methods for preparing acetylacetone. Compared with traditional chemical methods, biosynthesis is expected to have the advantages of environmental friendliness and mild conditions.

The biosynthesis pathway of acetylacetone was constructed by reversing its biodegradation pathway, and acetylacetone was successfully produced by overexpressing acetylacetone lyase (Dke1) of Acinetobacter yoelii in E. coli. Based on sequence alignment and structural analysis, several promising amino acid residues were selected for enzyme improvement, and the yield of acetylacetone was increased by site directed mutagenesis of Dke1. The acetylacetone production capacity of double mutant (k15q / a60d) was the highest, 3.6 times that of wild-type protein. Finally, the strain accumulated 556.3 ± 15.2 mg / L acetylacetone in batch fed fermentation under anaerobic conditions.