Ultrapure Agarose CAS 9012-36-6

A preparation method of low solidification temperature level agarose, consisting of the actions:

1. Dissolve 4.0 g of agarose powder in 150 mL of deionized water at 90 ° C to prepare an agarose option;
2. After the above-mentioned agarose option was naturally cooled to 82 ° C., the sodium hydroxide remedy of 0.9 mL of salt borohydride was added thereto to perform decrease response for 15 minutes. Throughout the response, the shade adjustment of the service was observed. When the shade altered from light eco-friendly to colorless and clear When fluid, the reduction reaction is terminated. In the salt hydroxide remedy of salt borohydride, the concentration of sodium borohydride is 4.4 mol/L, and also the solvent is 10.0-20.0 mol/L NaOH remedy;
3. Adding 40 mL of salt hydroxide remedy with a focus of 3.8 mol/L to the solution gotten symphonious (2) to accomplish alkalization therapy;
4. The solution of action (3) was quickly cooled to 5 ° C, and 20-60% (v/v) liquid remedy of ethylene oxide was slowly included dropwise within 60 min while stirring up until the concentration of ethylene oxide in the solution was 1.0 ～ 5.0 wt%, and after that stirred for 60min;
5. Home heating the option acquired in step (4) to 70 ° C., adding 3.0 mol/L acetic acid remedy dropwise while stirring to readjust the pH worth of the solution to 7.2;dding 40 mL of salt hydroxide remedy with a focus of 3.8 mol/L to the solution gotten symphonious (2) to accomplish alkalization therapy;
6. Adding warm isopropanol at 45 ° C to the solution symphonious (5) while mixing, and afterwards standing for 1.0-3.0 h for alcohol precipitation category;
7. The material gotten symphonious (6) goes through centrifugal purification to get a white precipitate, which is immersed with 60% quantity focus of isopropanol and also crushed, then washed 4 times with isopropanol, and squeezed as well as dried out to acquire a filter cake;
8. After those filter cake is put in an air drying stove at 55 ° C and also dried out at a constant temperature level for 12.0 h, after that crushed and also sieved with a 200-mesh filter to obtain the agarose of the low solidification temperature, and the melting temperature level of the agarose is 60.0 ℃, solidification temperature 26.5 ℃, gel toughness is 265g/cm2.