Guanidine Thiocyanate CAS 593-84-0 GITC

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  • Appearance: White powder
  • Assay: 99. 0%min
  • Stock: In stock
  • Sample: Available
  • Zhishang Chemical: Guanidine Thiocyanate Supplement

Basic Info of Guanidine Thiocyanate

What is Guanidine Thiocyanate?

Guanidine thiocyanate is also called Guanidinethiocyanatesalt, chemical name: imidothiocyanate, and its molecular formula is: (NH2)2C: NH·HSCN (118.2).

Guanidine isothiocyanate is in the form of white crystals, the melting point is 116~121℃, the pH value of 6mol/L in water is 4.5~6 at 20℃, and the solubility is completely dissolved. It is a kind of uncoupler and a kind of strong protein denaturant, which can dissolve protein, make protein secondary structure disappear, cell structure degrade, and nucleoprotein is rapidly separated from nucleic acid. For denaturing and lysing cells; extract RNA and DNA, without RNAse and DNAse activity.

Guanidine thiocyanate can be used as a pharmaceutical intermediate. It is used for degreasing in oil industry, as strong lipid degrading agent in daily chemical industry, and cell membrane rupture agent in biopharmaceutical.

Guanidine Thiocyanate Uses

  1. Guanidine thiocyanate mostly utilized for biomedicine, chemical reagents, etc.
  2. Chaotropic agent and solid denaturant for denaturing and lysing cells; extracting RNA and DNA.
  3. Guanidine thiocyanate is a denaturant typically utilized in RNA seclusion. It is utilized as a storage barrier for whole blood samples.Guanidine thiocyanate inactivates nucleases as well as is ideal for storing as well as cold stool examples for DNA research study. Used in mix with phenol-chloroform in RNA removal.
  4. Guanidine thiocyanate is used for degreasing in oil sector, as solid lipid degrading agent in daily chemical industry, and cell membrane layer rupture agent in biopharmaceutical.

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Application of Guanidine Thiocyanate

The Preparation Method of Guanidine Thiocyanate


  1. Guanidine Thiocyanate – PubChem
  2. CN201110146840.5 Total RNA preparation method without genomic DNA
  3. Hou Xiaofeng, Li Juan, Guo Guanghong.Methodological evaluation of the determination of lactate dehydrogenase isoenzyme 1 by guanidine thiocyanate inhibition method[J].Label Immunoassay and Clinical,2015,22(08):798-799+810.

Quality Control of Guanidine Thiocyanate

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