CHAPS CAS 75621-03-3
Factory Supply CHAPS CAS 75621-03-3 with Best Price
- Appearance: White powder
- Purity: 99. 0%min
- Stock: In stock
- Sample: Available
- Zhishang Chemical: CHAPS Supplier & Manufacturer
CHAPS: The Complete Guide
CHAPS for Sale
|Type:||Organic raw materials|
What is CHAPS?
CHAPS, a derivative of Cholic acid, is an amphoteric detergent that can dissolve membrane protein. CHAPS is used to stabilize various protein-DNA complexes and retain the biochemical activity of protein in solution.
CHAPS can be synthesized from cholic acid and is amphoteric due to its quaternary ammonium and sulfonate groups; It is similar in structure to some bile acids, such as taurine deoxycholic acid and taurine goose deoxycholic acid. Due to its natural hydrophobicity, it is slightly soluble or insoluble in aqueous solution.
CHAPS is a non-denaturing zwitterionic cleaning agent made use of in membrane layer biochemistry. Can be used to solubilize membrane layer healthy proteins and interrupt protein-protein interactions.
Men has a reduced micelle molecular weight (6,150) and also a high essential micelle focus (6-10 mM), so it can be gotten rid of from examples by dialysis. It is additionally suitable for solubilizing proteins in isoelectric focusing as well as 2D electrophoresis.
Men is frequently utilized in non-denaturing (urea-free) isoelectric focusing as well as has been found to offer outstanding resolution for certain subcellular preparations and also plant proteins. Concentrations in between 2-4% (w/v) are generally used for isoelectric concentrating gels.
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Application of CHAPS
CHAPS is usually used as a detergent in membrane protein solubilization and purification for several favorable reasons. CHAPS detergent will not denature membrane protein, can dissolve protein and decompose protein-protein interaction.
CHAPS can also be used for ion exchange chromatography and isoelectric focusing because it is a zwitterion and does not show net charge between pH 2 and 12. It is suitable for separating membrane proteins with the following characteristics, such as sufficient protein solubilization capacity, no denaturation or protein inactivation, no interference with protein activity, no precipitation at 4 ° C, appropriate critical micelle concentration (CMC) and micelle size, no absorption in UV region, non-toxicity and availability of detergent detection methods.
CHAPS Supplier and Manufacturer
As a CHAPS supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best CHAPS raw materials to customers all over the world.
Over the years, relying on professional team experience and customer-centric team concept to meet customer needs in a timely manner, our company enjoys a high reputation in the world. At present, our company has served more than 6,000 customers and has established long-term cooperative relations with customers from many countries.
If you have a demand for CHAPS and related products, please contact our service staff Zhishang Chemical – White directly, and we will provide you with high-quality products at the best price.
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